Free-radical scavenging activity on DPPHradical dot
The free-radical scavenging capacity of each extract was evaluated according to the procedure of Liyana-Pathirana and Shahidi (2007) with some modifications. Briefly, 100 μl of extract was added to the freshly prepared 0.1 mM DPPHradical dot solution (1.9 ml), and the mixture was kept at room temperature in a dark room for 30 min. The absorbance was read at 517 nm relative to the control (as 100%) and the percentage of scavenging effect was expressed as [1 − (A517 of sample/A517 of control)] × 100.